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. 2019 Jun 19;9:8742. doi: 10.1038/s41598-019-45009-1

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Pathology of microcystin exposure in liver. Formalin-fixed, paraffin-embedded 5 μm liver slices from lean mouse control (also denoted as CHOW) (N = 8), wild type mouse control (NAFLD) (N = 8), wild type mouse group exposed with Microcystin (CHOW + MC) (N = 8) and another NAFLD group of mice exposed with Microcystin (NAFLD + MC) (N = 8) were used for hematoxylin and eosin imaging. (A) Representative Hematoxylin and Eosin stained (H&E) images (20×) of liver sections showed NAFLD pathophysiology of control mice group, wild type NAFLD (WT-NAFLD), control group exposed to microcystin (CHOW + MC), and another group of NAFLD mice group exposed to Microcystin. (B) (i–iv) Representative Immunohistochemistry images (20×) depicting CD68 immuno-reactivity (C) Morphometric analysis of CD68 immunoreactivity (*p < 0.05).