Figure 6.

N‐[(3, 5‐Difluorophenyl) acetyl]‐L‐alanyl‐2‐ phenylglycine‐1, 1‐dimethylethyl ester (DAPT) inhibited the activation of the Notch1/NF‐κB and neuroinflammatory cytokines caused by folic acid deficiency (FD) and oxygen‐glucose deprivation (OGD). A, Western blot analysis of Notch1 and nuclear factor kappa B (NF‐κB) p65. B, Western blot analysis of pIkBα. C, Western blot analysis of interleukin‐1beta (IL‐1β), interleukin‐6 (IL‐6) and tumour necrosis factor‐α (TNF‐α). β‐actin protein was used here as an internal control. D, Nuclear factor kappa B p65 immunostaining of the BV‐2 cells. The data are presented as the mean ± SD (n = 4 each group). a P < 0.05 vs the OGD group, b P < 0.05 vs the OGD+FD group