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. 2019 Jun 19;9:8755. doi: 10.1038/s41598-019-45209-9

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Overexpression of PAR-4 sensitizes TNBC cells to DNA damage-induced cell death. (a) Lysates from a panel of breast cancer cell lines including MCF-7, T-47-D, ZR-75-1, SKBR-3 and triple negative breast cancer (TNBC) cell lines MDA-MB-468, MDA-MB-231, HS-578-T and BT-20 were analysed by immunoblotting using antibodies against PAR-4, caspase-8 and GAPDH and compared with the immortalised breast epithelial cell line MCF-10A. Expression status of estrogen receptor (ER), progesterone receptor (PR) and amplification of HER2/neu (HER2) is depicted for each cell line. PAR-4 expression was also quantified with ImageJ (bottom panel). (b) BT-20 and MDA-MB-468 cells were transfected with control vector or PAR-4 wild-type (WT) for 24 h. Cell lysates were analysed by antibodies against PAR-4 and GAPDH. (Upper panel) Cells were transfected as in the upper panel, incubated for 24 h, stimulated with Etoposide (100 μM) for 24 h and the protein lysates were analysed with the indicated antibodies (Lower panel). (a,b) Full-length blots are presented in Supplementary Fig. S3.