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. 2019 Jun 19;9:8755. doi: 10.1038/s41598-019-45209-9

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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PAR-4 is required for DNA damage-induced apoptosis in TNBC cells. (a) PAR-4 expression was silenced in BT-20 and MDA-MB-468 cells by using two PAR-4 specific siRNAs (siPAR-4 #1 and #2), and a scrambled siRNA as a control (siControl). Knockdown efficiency was analysed 48 h after transfection. (Left panel) After 48 hours, cells were stimulated with Etoposide (100 μM) for 16 h and cell lysates were analysed by immunoblotting using the indicated antibodies. (Right panel) (b) BT-20 and MDA-MB-468 cells from (a) were prepared for cell cycle analysis and the sub-G1 population was measured via flow cytometry. Average values from three independent experiments are shown. Error bars indicate ± SD. P-value was obtained by two-tailed Student’s t-Test. (a) Full-length blots are presented in Supplementary Fig. S3.