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. 2019 Jun 19;9:8755. doi: 10.1038/s41598-019-45209-9

Figure 3.

Figure 3

Caspase-8 activation is required for DNA damage-induced cell death. (a) BT-20 and MDA-MB-468 control (shcontrol) or caspase-8-deficient (shcaspase-8) cells were treated with Etoposide (100 μM) for 24 h and lysates were analysed with the indicated antibodies. (b) TNBC cells as in (a) were treated with Doxorubicin (10 μM) for 8 h and analysed as described under (a). (c) TNBCs from (a) were prepared for cell cycle analysis and the sub-G1 population was measured via flow cytometry. Error bars indicate ± SD. (d) BT-20 and MDA-MB-468 cells were pre-incubated with the caspase-8 inhibitor Z-IETD-FMK (20 μM) for 30 min followed by treatment with Etoposide (100 μM) for 24 h. Lysates were analysed via immunoblotting with the indicated antibodies. (e) MCF-7 cells were pre-incubated with the caspase-8 inhibitor Z-IETD-FMK (20 μM) for 30 min followed by treatment with Doxorubicin (10 μM) for 16 h or with Etoposide (100 μM) for 24 h. Lysates were analysed via immunoblotting with the indicated antibodies. (a,b,d,e) Full-length blots are presented in Supplementary Fig. S4.