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. 2019 Jun 19;9:8755. doi: 10.1038/s41598-019-45209-9

Figure 6.

Figure 6

Depletion of cIAP1 using Smac Mimetic (LC161) or siRNA, sensitizes TNBCs to DNA damage-induced apoptosis in drug resistant PAR-4 deficient cells. (a) Par-4 expression in the indicated TNBC cells was silenced as shown in (Fig. 2a). Cells were pre-treated with cIAP1 antagonist LCL161 (0,5 μM) for 1 h and then stimulated with Etoposide (100 μM) for 16 h. Cell lysates were analysed by immunoblotting using the indicated antibodies. (b) TNBCs from (a) were prepared for cell cycle analysis and the sub-G1 population was measured via flow cytometry. The corresponding histograms are presented in Supplementary Fig. S2. Average values from three independent experiments are shown. Error bars indicate ± SD. (c) PAR-4 and cIAP1 expression were silenced in BT-20 and MDA-MB-468 cells by using specific siRNAs (siPAR-4 #1, siPAR-4 #2 and sicIAP1). After incubation for 48 h cells were stimulated with Etoposide (100 μM) for 16 h and cell lysates were analysed by immunoblotting using the indicated antibodies. (a,c) Full-length blots are presented in Supplementary Fig. S7.