. 2019 May 18;23(7):4611–4626. doi: 10.1111/jcmm.14341

Table 1.

Primer sequences for qRT‐PCR analysis

Gene	Primer sequence (5′→3′)	Length (bp)	Annealing temperature
PCNA	Forward: 5′‐GAAGTTGGAGGCACTCAAGG‐3′	145	61.5°C
PCNA	Reverse: 5′‐GCAGCGGTAGGTGTCGAAGCC‐3′_	145	61.5°C
p27	Forward: 5′‐GCAAGTACGAGTGGCAAGAGGTG‐3′	118	62.0°C
p27	Reverse: 5′‐CCGCTGACATCCTGGCTCTCC‐3′	118	62.0°C
MFN2	Forward: 5′‐GGTGCTCAACGCCAGGATTCAG‐3′	197	61.5°C
MFN2	Reverse:5′‐GTCGAACCGCCTCTGCAATCTG‐3′	197	61.5°C
DNMT1	Forward: 5′‐ATCGAGACCACGGTTCCTCCTTC‐3′	101	61°C
DNMT1	Reverse: 5′‐TAACTCTCCTGCTCCACCAC‐3′	101	61°C
c‐Myc	Forward: 5′‐CGAGGAGAATGTCAAGAGGCGAAC‐3′	174	60.5°C
c‐Myc	Reverse: 5′‐GCTTGGACGGACAGGATGTATGC‐3′	174	60.5°C
GAPDH	Forward:5′‐AGAAGGCTGGGGCTCATTTG‐3′	258	58°C
GAPDH	Reverse:5′‐AGGGGCCATCCACAGTCTTC‐3′	258	58°C