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. 2019 Jun 19;9:8725. doi: 10.1038/s41598-019-44259-3

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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VIPP1 forms large rods that can contain lipids. Negative stain electron micrographs of (a) VIPP1 alone, (b) VIPP1 incubated with PC liposomes and (c) VIPP1 incubated with PC:PI4P (95:5) liposomes. The same VIPP1 preparation (0.4 mg/mL, Prep #4, see methods) was used for all conditions, and this sample was also used for the “VIPP1 + PC:PI4P” cryo-ET in Figs. 3 and 4 (see methods). Encapsulated distinctly stained material inside the VIPP1 rods is indicated with arrowheads. (d) Distribution of rod diameters for the three conditions in (a–c). (e) Distribution of rod diameters for VIPP1 alone for two different VIPP1 preparations at concentrations of 0.4 mg/mL and 0.7 mg/mL (Preps #2 and #1, respectively, see methods). The 0.4 mg/mL sample was also used for the “VIPP1 alone” cryo-ET in Figs. 3 and 4.