Table 2.
List of primary antibodies, secondary antibodies and blocking buffer used for western blot analysis are listed.
| Target | Blocking buffer | Antibody | Concentration | Species | Reference |
|---|---|---|---|---|---|
| Cx37 | 5% NFDM | Primary | 1/500 | Rabbit | Life Technologies (Belgium) |
| Secondary | 1/10 000 | Goat | Life Technologies (Belgium) | ||
| Cx40 | 5% BSA | Primary | 1/500 | Goat | Santa Cruz (Germany) |
| Secondary | 1/10 000 | Rabbit | Life Technologies (Belgium) | ||
| Cx43 | 5% NFDM | Primary | 1/1 000 | Rabbit | Sigma-Aldrich Co.(Belgium) |
| Secondary | 1/20 000 | Goat | Life Technologies (Belgium) | ||
| Vinculin | 5% NFDM | Primary | 1/1 000 | Mouse | Santa Cruz (Germany) |
| Secondary | 1/20 000 | Goat | Life Technologies (Belgium) |
Cx: connexin, NFDM: non-fat dry milk, BSA: bovine serum albumin.