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. 2019 Jun 19;9:8771. doi: 10.1038/s41598-019-43998-7

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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AICAR treatment of immortalized keratinocytes NTERTs (NT) and dermal papilla (DP) in vitro for 24 hours. PGC1α expression level in (A) NT, (p = 0.005) and (B) DP cells (p = 0.06) by qPCR compared to untreated control. EdU positive cells by flow cytometry after EdU labelling for 2 hours of AICAR treated (C) NT and (D) DP cells compared to untreated control. Data represented as mean ± SD. n = 3 per group. p-value for difference between untreated and treatment group: NTERTs p < 0.0001, DP p = 0.043.