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. 2019 Jun 19;9:8801. doi: 10.1038/s41598-019-45238-4

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Viability of mesenchymal stem cells cultured on the top of silk fibroin hydrogels. (a) Representative images of calcein (green)- and propidium iodide (red)-positive MSCs at different time points after seeding on the top of plastic (TCP) or silk fibroin (2%) hydrogels (Scale bar: 500 μm). (b) Representative images of MSCs adhered on the top of silk fibroin hydrogels (Scale bar: 50 μm). Although the survival of MSCs was drastically reduced, some cells could be visualized exhibiting attachment and extending cellular processes (black arrows).