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. 2019 Jun 19;9:8801. doi: 10.1038/s41598-019-45238-4

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Proliferation of Mesenchymal stem cells in silk fibroin films. (a) Representative images of MSCs growing on plastic (TCP) and silk fibroin (SF) at different time points after seeding (scale bar: 200 μm). (b) Cell fold expansion over TCP or SF after time in culture. (c) Percentage of cells in each cell cycle phase (G₀/G₁, S and G₂/M) across time in culture. The data in (b,c) are shown as mean ± the SEM of three independent experiments in triplicate (9 samples in total per group and temporal point).