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. 2019 Jun 19;9:8801. doi: 10.1038/s41598-019-45238-4

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Short- and long-term viability studies of mesenchymal stem cells in contact with silk fibroin films. (a) Representative flow cytometry dot plots to illustrate the relationship between propidium iodide fluorescence intensity (a marker of late apoptotic and necrotic cells) and cellular size (Forward Scatter) in MSCs over TCP or silk fibroin. (b) Percentage of dead cells across time after seeding. The insets in the top part show representative microscopy images of MSCs grown over TCP or silk fibroin at seven weeks of culture (Scale bar: 100 μm). The data are shown as mean ± the SEM of three independent experiments with 9 samples per group and temporal point.