Fig. 4. FBXO22 mediates non-degradative polyubiquitination of LKB1.

293T (a), H322, and H1299 (b) cells were infected with shNC or shFBXO22, cell lysates were analyzed by western blots for the indicated proteins. c Cell lysates from wild-type and FBXO22 knockout mice MEF cells were subjected to western blots to detect indicated proteins. 293T (d), H322 and H1299 (e) cells were transfected with FBXO22 and ΔFBXO22, cell lysates were analyzed by western blots for the indicated proteins. f LKB1-deficient H460 and A549 cells were transfected with Flag-LKB1 and FBXO22, cell lysates were analyzed by western blots for the indicated proteins. H1299 cells expressing the Doxycycline (Dox) inducible FBXO22 were treated with Dox for different time and concentration (g), the same cells were treated with or without Dox (200 ng/ml) for different time (h), cell lysates were analyzed by western blots for the indicated proteins