Table 2.

Development of microcirculatory parameters across induction of septic shock and resuscitation as reflected by manual analysis as well as using an advanced computer vision algorithm

		All individuals	Septic shock group
			Baseline		Septic shock			Resuscitation
		n = 17	n = 10		n = 10			n = 5
		Mean ± SD	Mean ± SD	Mean ± SD	Estimate ± SE (CI)	t statistic	Mean ± SD	Estimate ± SE (CI)	t statistic	p
Total vessel density (capillaries)
TVD (manual) [mm mm−2]		18.9 ± 3.9	19.7 ± 3.9	17.6 ± 5.2	−1.7 ± 1.1 (−3.7 to 0.2)	−1.53	16.9 ± 3.4	0.7 ± 1.0 (−0.9 to 2.4)	0.76	0.18
TVD (algorithm) [mm mm−2]		19.9 ± 4.1	22.3 ± 4.6	20.5 ± 4.6	−1.0 ± 1.5 (−3.4 to 1.5)	−0.66	20.9 ± 2.9	0.9 ± 1.3 (−1.2 to 3.1)	0.74	0.54
Red blood cell flow velocity (capillaries)
MFI (manual) [1]		2.7 ± 0.5	2.9 ± 0.3	2.2 ± 0.5	−0.5 ± 0.2 (−0.8 to −0.2)	−2.62	2.2 ± 0.8	0.2 ± 0.2 (0.0–0.5)	1.55	0.01
RBCv (algorithm) [µm s−1]		232 ± 75	270 ± 78	165 ± 67	−73 ± 21 (−110 to −37)	−3.41	163 ± 75	44 ± 18 (41–57)	2.36	<0.001
Functional capillary density
FCD (manual) [mm mm−2]		16.6 ± 4.1	18.0 ± 3.4	13.4 ± 3.9	−4.0 ± 1.3 (−6.2 to −1.8)	−3.11	12.3 ± 3.0	1.5 ± 1.1 (−0.5 to 3.4)	1.30	<0.01
PPV (manual) [1]		88 ± 10	92 ± 6	77 ± 11	−13 ± 4 (−19 to −7)	−3.55	74 ± 13	5 ± 3 (−1 to 10)	1.48	<0.01
FCD (algorithm) [mm mm−2]		16.7 ± 4.2	20.1 ± 4.0	14.8 ± 4.9	−3.9 ± 1.5 (−6.5 to −1.3)	−2.57	14.6 ± 2.5	2.1 ± 1.3 (−0.1 to 4.4)	1.60	<0.01
PPV (algorithm) [mm mm−2]		85 ± 13	91 ± 7	73 ± 18	−14 ± 5 (−22 to −6)	−2.98	71 ± 15	6 ± 4 (0–13)	1.56	<0.01

Timepoints were compared using a one-way mixed linear model analysis with timepoint as fixed effects and subject number as random effects. Estimates, standard error, confidence intervals, and t statistic are given for each timepoint. Full model p values were computed calculated using a likelihood ratio test. TVD total vessel density, FCD functional capillary density, PPV proportion of perfused vessels, MFI microvascular flow index, RBCv red blood cell velocity, SD standard deviation, SE standard error, CI confidence interval