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. 2019 Jun 19;10(7):484. doi: 10.1038/s41419-019-1714-y

Fig. 4. LZ-101 stabilized FOXO3a proteins in a proteasome-independent manner in A549 cells.

Fig. 4

a Degradation dynamics of FOXO3a following a time course CHX treatment in A549 cells treated with 15 μM LZ-101. b FOXO3a proteins were stabilized in the presence of MG132 (20 μM) in A549 cells treated with 15 μM LZ-101. c Annexin-V/PI staining assay measured by flow cytometry in the presence of MG132 (20 μM) in A549 cells treated with 15 μM LZ-101. d Confocal microscopy of A549 cells treated with 15 μM LZ-101 or 100 nM rapamycin, immunostained for FOXO3a (green) and LAMP-1 (red). Scale bars, 10 µm (top). Histograms shows the co-localized pixels of FOXO3a and LAMP1 per cell for three independent experiments (mean ± SD) (bottom). e FOXO3a was detected by Western blot after 15 μM LZ-101 and 30 nM bafilomycin A1 treatment. Data are presented as mean ± SD. *P < 0.05, **P < 0.01 compared with DMSO group; #P < 0.05, ##P < 0.01