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. 2019 Jun 19;9:8745. doi: 10.1038/s41598-019-45166-3

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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The experiment with four-channel flow cell. (a) Parallel detection of three soluble tumor markers in a four-channel flow cell containing antibodies against CA125, HER2, and CA15-3. The dip in the signal corresponding to CA125 detection (dashed line) is caused by an air bubble formation inside of the flow cell. (b) A signal corresponding to CA125 detection. (c) A signal corresponding to HER2 detection. (d) A signal corresponding to CA15-3 detection. (e) A schematic diagram of the four-channel flow cell used in the experiment.