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. 2019 Jun 19;9:8745. doi: 10.1038/s41598-019-45166-3

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Quantitative detection of HER2 antigen using photonic crystal surface mode detection. (a) The dependence of the adlayer thickness increase and decrease on the HER2 concentration; after the start of the HER2 flow, the signal was allowed to reach a plateau (pre-washing adlayer thickness); then, the solution was switched to PBS to begin the washing stage; the washing stage was marked with a rapid decrease in the signal intensity until it reached a new plateau (post-washing adlayer thickness). (b) The linear dependence of the pre-washing adlayer thickness on the HER2 concentration. (c) The linear dependence of the post-washing adlayer thickness on the HER2 concentration (the value before HER2 binding taken to be the zero point).