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. 2019 Jun 19;5(6):eaav8062. doi: 10.1126/sciadv.aav8062

Fig. 1. Correlative imaging.

Fig. 1

(A) Images of the same area are acquired in different modes. The confocal and STED images are acquired in reflection, while the AFM probe reaches the sample from above, providing a three-dimensional topographical view at high resolution. (B) Amyloid fibrils from bovine insulin labeled with fluorescence dye ATTO 488 NHS ester with a 1:19 dye-to-protein ratio. The STED and confocal images are overlaid with AFM topography. The resolution of the STED microscopy image is significantly enhanced with respect to the confocal microscopy image. At the same time, the AFM provides the topographical image on the same sample area. Some fibrillar aggregates are not displayed in fluorescence microscopy (e.g., white arrow). Scale bar, 1 μm.