Figure 6.

Ablation of MFS2 and overexpression of bnl in principal cells decreases or increases Malpighian tubule deposits, respectively. (A) Flies expressing the tubule driver Uro-Gal4^ts and RNAi targeting white (w) as control and MFS2 (B), or a transgene (TG) for bnl (C,D) were generated and cultured on control medium at 29 °C to induce gene expression for 20–30 days as shown in the scheme, followed by dissection and complete lysis of anterior and posterior Malpighian tubule pairs for calcium assay (C), or RNA preparation followed by qRT-PCR for MFS2 (D) (mean ± SEM, n = 4–5, **p = 0.002, ***p = 0.0002 vs. white RNAi).