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. 2019 Jun 19;9:8760. doi: 10.1038/s41598-019-45114-1

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Confocal microscopy analysis of caveolin-1 (CAV-1) distribution. (Panel A) Normal liver (a) exhibits lack of CAV-1 expression in hepatocytes, while positive staining is restricted to the sinusoidal endothelial cells surface. In HCV-infected liver (b,c) the labeling is present both on hepatocytes and endothelial cells. (Panel B) Representative confocal images showing different staining pattern into parenchymal cells: the staining was found as absent in the nucleus and diffuse in the cytoplasm (I), or positive in the nucleus and present as puncta in the cytoplasm (II). Scale bar; A (a–c) = 25 μm; and B (I,II) = 12 μm.