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. 2019 Jun 12;10:1315. doi: 10.3389/fmicb.2019.01315

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Copyright © 2019 Babele, Kumar and Chaturvedi.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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A typical workflow of comparative proteomic analysis in cyanobacteria. Proteins are extracted, cleaned and subjected to separation via gel (2-Dimensional gel electrophoresis) or non-gel (liquid chromatography) based approaches. Reduction, alkylation and trypsin digestion are performed before or after the separation step as per the requirement to convert protein mixtures into peptides. Separated peptides are analyzed through mass spectrometer (MS) followed by tandem MS (MS/MS) for the protein identification. The detected protein list is then used for data analysis using various bioinformatic tools.