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Schematic flow of this work. General schematic of the expression and purification of Ydj1, as well as the zinc removal procedure. First, cell culturing was performed to express Ydj1. Then, Ydj1 was isolated free of contaminants using liquid chromatography. Then, an optimization methodology was performed to remove Zn from Ydj1. ICP-MS was used for Zn quantification analysis and to evaluate the efficiency of Zn removal.
