Figure 3.

Time course of the effects of a single subcutaneous (s.c.) injection of S1RA (8–128 mg/kg) or saline on mechanical allodynia (A), heat hyperalgesia (B), and cold allodynia (C), in female WT mice with SNI, 7 days after surgery. The von Frey threshold (A), the latency to hind paw withdrawal in the Hargreaves test (B), and the duration of hind paw licking or biting in the acetone test (C) were recorded 1 day before (baseline, BL) and 7 days (Day 7) after surgery in the paw ipsilateral to the surgery. On day 7, the responses to test stimuli was recorded immediately before (time 0) and at several times (30, 90, and 180 min) after injection of the drug or saline. Each point and vertical line represent the mean ± SEM of the values obtained in 8–11 animals. Statistically significant differences between S1RA- and saline-treated groups at the same time after treatment: **P < 0.01 (two-way repeated-measures ANOVA followed by Bonferroni test).