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. 2019 Apr 23;32(6):1103–1114. doi: 10.1021/acs.chemrestox.8b00412

Figure 3.

Figure 3

Percentage (±SD, n = 3) of BAC recovered from exposure medium after 48 h of exposure to RTgill-W1 in 96-well plates. Panels (a)–(c), respectively, depict the percentages recovered from L15/ex, L15/ex with 4 g/L BSA, and L15/ex with 10% FBS. The recovered percentages are sorted by the concentration at the start of the exposure (from low (C1) to high(C9), white to black bars). The concentrations are 0.01–25 μM in L15/ex for BAC10-BAC18, 0.04–50 μM for BAC10-BAC18 in L15/ex with medium constituents, 0.2–250 μM for BAC8, and 0.5–500 μM for BAC6 in L15/ex or 0.5–1000 μM for BAC8 and 1.5–1000 μM for BAC6 in L15/ex with medium constituents. The percentage of chemical not recovered from medium after exposure is assumed to have sorbed to plastic and cells. Note that the red stripes below the bars refer to recoveries calculated using estimated concentrations after exposure. Actual concentrations could not be established because they were below the limit of quantification. Estimations were based on dilution factors.