Figure 4.

Knockdown of CBX2 increases the apoptosis of human HCC cells in vitro. (A, C) Knockdown of CBX2 increases apoptosis of Huh7 and Bel 7402 cells. Apoptotic cells were detected by Annexin V-PI dual staining. Representative data from three independent experiments are shown. The total number of cells in the Q2 and Q4 quadrants was regarded as apoptotic cells. (B, D) Percentages of apoptotic Huh7 and Bel 7402 cells are shown in the bar graph (right panel). The data are the means ± SD of three independent assays (** P < 0.01).