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. 2019 Jun 2;10(12):2706–2719. doi: 10.7150/jca.31845

Figure 5.

Figure 5

Knockdown of CBX2 inhibits WTIP and increases the phosphorylation of YAP. (A) RNA-seq showed some pathway enrichment differences between the CBX2 knockdown group and the control group according to the KEGG database. (B) Heatmap of the RNA differentiation cluster between the CBX2 knockdown group and the control group (the result shown in this figure is a portion of the entire heatmap). Compared with the control group, the RNA level of WTIP decreased significantly in the CBX2 knockdown group (P<0.05). (C) CBX2 protein was decreased in LV3-Si3 cells. CBX2, WTIP, YAP, P-YAP, AREG and GLI2 protein levels in the LV3-ctl and LV3-Si3 groups of Huh7 and Bel7402 cells were determined using western blot. Band intensities were quantified using Image Lab 5.0 software and were normalized to GAPDH. The data are presented as the mean ± SD of three separate experiments (*P<0.05). (D) CBX2 protein was increased in LV5-CBX2 cells. CBX2, WTIP, YAP, P-YAP, AREG and GLI2 protein levels in the LV5-ctl and LV5-CBX2 groups of Huh7 and Bel7402 cells were determined using western blot. Band intensities were quantified using Image Lab 5.0 software and were normalized to GAPDH. The data are presented as the mean ± SD of three separate experiments (*P<0.05).