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. 2019 Jun 2;10(12):2706–2719. doi: 10.7150/jca.31845

Figure 6.

Figure 6

Further prove knockdown of CBX2 inhibits WTIP and increases the phosphorylation of YAP. (A) Overexpression of WTIP in CBX2 knockdown Huh7 cells. CBX2, WTIP, YAP and P-YAP protein levels in the test and NC groups were determined using western blot. Knockdown of WTIP in CBX2-overexpressing Huh7 cells. CBX2, WTIP, YAP and P-YAP protein levels in the test and NC groups were determined using western blot. Band intensities were quantified using Image Lab 5.0 software and were normalized to GAPDH. The data are presented as the mean ± SD of three separate experiments (*P<0.05). (B) Overexpression of WTIP in CBX2 knockdown Huh7 cells (G2) and just CBX2 knockdown Huh7 cells (G1); knockdown of WTIP in CBX2-overexpressing Huh7 cells (G4) and just CBX2-overexpressing Huh7 cells (G3). Overexpression of WTIP reduced the increased apoptosis caused by knockdown of CBX2 in Huh7 cells. Knockdown of WTIP increased the apoptosis of Huh7 cells. Apoptotic cells were detected by Annexin V-PI dual staining. Representative data from three independent experiments are shown. The total number of cells in the Q2 and Q4 quadrants was regarded as the total apoptotic cells. (C) Percentages of apoptotic Huh7 cells are shown in the bar graph. The data are the means ± SD of three independent assays (** P < 0.01).