Fig 1.

p16/Ki-67 dual-staining positive cells with morphological features of HSIL. A, Liquid-based cytology (SurePath). B, Slide A was dual-stained with p16/Ki-67. Cell with p16 staining alone (blue arrow) is characterized by a brown cytoplasmic/nuclear signal and cell with Ki-67 staining alone (red arrow) is presented in red nuclear signal. The positive p16/Ki-67 dual-staining cells (dark arrow) are characterized by a brown cytoplasmic signal for p16 overexpression and a dark red nuclear signal for p16/Ki-67 co-expression in the same cell. (45-year-old woman, CIN3, HPV16+, p16/Ki-67+).