Fig 5.
Effects of ubiquitin on tumor metastasis, body weight and survival rate in tumor-bearing mice. (A) B16-luciferase cells (5×105) were inoculated into the tail vein of mice, and these mice were randomly divided into three groups (n=6, each group): intraperitoneally injected with 200 μl of normal saline, 320 ng of ubiquitin and 3 200 ng of ubiquitin, respectively. The luciferase activity of tumor cells was monitored by real-time imaging on the 9th, 14th and 19th day after inoculation. (B) The statistical analysis of in vivo luciferase activity. (C) The analysis of the rate of weight gain in each group of tumor-bearing mice. (D) Log-rank survival analysis of tumor-bearing mice in the three groups (control, 320 ng and 3 200 ng ubiquitin intervention groups). Data obtained from three independent experiments were expressed as mean ± standard error of the mean (SEM). * represents a P-value of <0.05; ** represents a P-value of <0.01.