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. 2019 Jun 2;10(12):2720–2734. doi: 10.7150/jca.31825

Figure 1.

Figure 1

Downregulation of miR-519d in OSCC indicates malignant transformation. (A) The expression level of miR-519d in 60 pairs of OSCC tissues and adjacent normal tissues (P<0.0001). (B, C) Low miR-519d expression was correlated with lymph node metastasis (P=0.037) and advanced TNM stage (P=0.001) in OSCC. (D) Kaplan-Meier analyses of overall survival. Patients with low miR-519d expression had a significantly lower overall survival rate than patients with high miR-519d expression (P=0.0006). (E) The miR-519d expression in normal oral epithelial cells, primary cultured cancer cells and 4 OSCC cell lines. (F) The methylation status of the DNA region located upstream of C19MC was detected in 5 OSCC cell lines and 24 pairs of OSCC samples and adjacent normal tissues by MSP. OSCC, oral squamous cell carcinoma; NC, matched adjacent normal tissue. (G, H) The expression of miR-519d in OSCC cells treated with or without the demethylating agent 5-Aza-dc (10 μM) for 72 h. **, P < 0.01. ***, P< 0.001. ****, P<0.0001.