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. 2019 Jun 2;10(12):2720–2734. doi: 10.7150/jca.31825

Figure 3.

Figure 3

MMP3 is a direct target of miR-519d in OSCC. (A) Venn diagrams of the predicted candidate target genes of miR-519d by bioinformatic method (miRecords) and mRNA microarray (GSE30784). (B) qPCR was adopted to validate the 13 potential targets in HN4 or HN30 cells after the transfection of miR-519d mimics or miR-NC. (C) The mRNA expression level of miR-519d in OSCC cells transfected with or without miR-519d inhibitor. (D) The protein level of MMP3 in HN4 or HN30 cells at 72 h after the transfection of miR-519d mimics or miR-519d inhibitor, as suggested by western blotting. (E) The predicted binding site of miR-519d and MMP3 and detailed sequences of WT-3′-UTR or MUT-3′-UTR of MMP3. (F, G and H) Luciferase reporter activity revealed the relative MMP3 reporter activities in 293T, HN4 or HN30 cells co-transfected with miR-519d mimics plus luciferase reporters or miR-519d inhibitor plus luciferase reporters. *, P < 0.05, **, P < 0.01. ***, P< 0.001. ****, P<0.0001