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. 2019 Jun 2;10(12):2720–2734. doi: 10.7150/jca.31825

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Regulation of MMP3 influences the effect of miR-519d on the migration and invasion of OSCC cells. (A) The mRNA and protein expression of MMP3 in HN4 or HN30 cells after concurrent transfection with miR-519d or/and an exogenous MMP3 expression vector. (B) The mRNA and protein expression of MMP3 in OSCC cells were detected after the transfection of miR-519d inhibitor or/and MMP3 specific siRNA. (C) Wound healing assay revealed that miR-519d inhibited the migration of HN4 or HN30 cells while ectopic expression of MMP3 reversed this effect. (D) As presented by wound healing assay, inhibiting the expression of MMP3 by siRNA could rescue the miR-519d inhibitor-induced promotion of cell migration. (E) Transwell invasion assay performed in HN4 or HN30 cells showed that the miR-519d-induced inhibition of cell invasion was abolished by MMP3 transfection. (F) siRNA targeting MMP3 rescued the tumor promoting effect of miR-519d inhibitor on cell invasion in OSCC. *, P < 0.05, **, P < 0.01. ***, P< 0.001. ****, P<0.0001.