Fig. 2.
Generation of PD-L1241-265 peptide-specific CD4+ T-cells. a Two CD4+ T-cell lines (G1 and G2) specific for PD-L1241-265 peptide were evaluated for their IFN-γ production in response to irradiated autologous PBMCs in the presence of various concentration of PD-L1241-265 peptide. b HLA restriction of each PD-L1241-265 -reactive CD4+ T-cell line was evaluated by using anti-HLA-DR mAb L243 and anti-HLA class I mAb W6/32 (negative control). c Each CD4+ T-cell line was cocultured with PD-L1241-265 peptide-pulsed L-cells expressing individual HLA-DR allele. Supernatants were collected after 48 h and analyzed for IFN-γ release by ELISA. Bars and error bars indicate the mean and SD of triplicate determinations, respectively. Each data is representative of two separate experiments