Fig. 4.

Effect of CA treatment on nuclear factor-E2 p45-related factor (Nrf2) gene expression and on protein nuclear translocation in HepG2 cells. Except for the control, in which cells were not exposed to any chemical, cells were incubated with CA (5, 10, and 20 μM) — or not at all — for 24 h and before being incubated with 0.3 mM t-BHP for 2 h. a Total RNA was extracted using the Trizol reagent and equivalent amount of RNA was converted into cDNA with the reverse transcriptase kit according to manufacturer’s instructions. RT-PCR and qRT-PCR experiments were performed to determine the amount of Nrf2 mRNA in HepG2 cells. b Proteins (10 μg) were separated by 10% SDS-PAGE and electro-transferred to PVDF membrane. Values are expressed as mean ± standard deviation (n = 3). Different letters indicate signification differences at p < 0.05 by Tukey’s studentized range tests