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. 2019 Jun 20;19:139. doi: 10.1186/s12906-019-2551-3

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 8 — Effect of CA treatment on DNA-binding activity of ARE in HepG2 cells. Cells were treated with 0.1% DMSO (lanes 2 and 4) and with the indicated concentration of CA (lanes 5–7) for 24 h. After treatment with CA, HepG2 cells were incubated with a 0.3 mM concentration of t-BHP for 2 h. Nuclear proteins (8 μg) were isolated, incubated with a labeled ARE sequence and subjected to electrophoretic mobility shift assay. A competition experiment (200-fold excess of unlabeled ARE) was performed with nuclear protein extracted from the non-treated control (lane 2)