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. 2019 Jun 20;13:55. doi: 10.1186/s13036-019-0177-0

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 7 — MC-coated hydrogel dishes for hESCs differentiation. a Original photograph s of the MC Hydrogel-coated in a polystyrene dish at distinct temperatures; b Photograph of a water drop on the surface of the MC hydrogel coated in a polystyrene dish in the dried or hydrated state; c Photomicrographs of the hESCs cultivated by different methods for distinct periods (magnification 40x). MC: methyl cellulose; hESCs: human embryonic stem cells; HDC: hanging drop culture; LSC-PS: liquid suspension culture in polystyrene dish; LSC-ULAP: liquid suspension culture in the Corning Ultralow Attachment plate; LSC-MC/PS: liquid suspension culture in the MC-coated polystyrene dish. Scale bars, 1.0 mm [124, 134]