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. 2019 Jun 13;9:209. doi: 10.3389/fcimb.2019.00209

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Copyright © 2019 Chen, Yu, Feng, Gan, Xie, Zhang, Xie, Wang, Ran, Zhang, Xiong and Shao.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Detection of Mhp Eno on the surface of M. hyopneumoniae by flow cytometry and immune electron microscopy. (A) Blank control, M. hyopneumoniae strain 168 treated with PBS; negative control, M. hyopneumoniae strain 168 treated with preimmune serum; M. hyopneumoniae strains 168, NJ, and WX treated with anti-Mhp Eno serum. (B) The MFI level of M. hyopneumoniae incubated with anti-Mhp Eno sera is expressed as the percentage of that found for the corresponding strain incubated with preimmune sera. The asterisks above the charts indicate statistically significant differences. (C) From left to right, the first three M. hyopneumoniae strains, 168, NJ and WX, were treated with anti-Mhp Eno serum and secondary gold-conjugated antibodies. The last column indicates the M. hyopneumoniae strain treated with preimmune serum and secondary gold-conjugated antibodies.