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. 2019 Jun 3;28(11):717–733. doi: 10.1089/scd.2018.0194

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© Nikola Sobočan et al. 2019; Published by Mary Ann Liebert, Inc.

This Open Access article is distributed under the terms of the Creative Commons License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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FIG. 5. — PCNA expression (arrows) in hindlimb buds of embryos from dams previously treated with 5azaC and/or PBN, detected by immunohistochemistry: (A) control; (B) PBN-treated; (C) 5azaC-treated; (D) PBN-pretreated and 5azaC-treated forelimb bud. DAB, hematoxylin counterstain. Thick arrows point to internal negative controls. Quantification of PCNA-positive signals (E) by the stereological measurement of numerical density (N_v). N_v values (columns) represent mean ± SEM. ANOVA, Holm–Sidak's multiple-comparison post hoc test. *P < 0.05, **P < 0.001, and ***P < 0.0001. Color images are available online.