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(Immuno)staining of the collagen implants (cell-free) after placement into the inner defect of the cartilage rings and subsequent culture for 0, 4, 8, 10, or 12 weeks. Safranin O staining and immunostaining for aggrecan, collagen 2, and collagen 1 (for quantification see Fig. 6 ); staining with (isotype-matched) control immunoglobulins consistently yielded negative results.
