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. 2019 May 9;8(10):e012327. doi: 10.1161/JAHA.119.012327

Figure 6.

Figure 6

The expression of miR‐145 (A) and miR‐143 (B) in vascular smooth muscle cells (VSMCs). VSMCs were obtained from the aortas of 3‐week‐old male Wistar‐Kyoto (WKY) rats, spontaneously hypertensive rats (SHRs), and complement 3 knockout (C3KO) SHRs by the explant method. The effects of miR‐143 and miR‐145 inhibitors on the expression C3 mRNA in VSMCs from WKY rats (C) and SHRs (D). The effects of miR‐143 and miR‐145 inhibitors on the expression of Krüppel‐like factor 5 (KLF5) mRNA in VSMCs from WKY rats (E), SHRs (F), and C3KO SHRs (G). VSMCs (105 cells/cm2) were transfected with miR‐145 inhibitor or miR‐143 inhibitor using lipofectamine. Two days later, total RNA was extracted, and real‐time PCR was performed. Significance was determined using 1‐way ANOVA, followed by Bonferroni post hoc test. Comparisons between 2 groups were assessed by using Student t test. Data are the mean±SEM (n=4–6). *P<0.05, **P<0.01 between indicated columns.