Figure 7.

Interaction of FSP27 and VEGF‐A. A, Co‐immunoprecipitation of FSP27 followed by immunoblot of VEGF‐A in cultured human aortic endothelial cells demonstrating an interaction between FSP27 and VEGF‐A. B, In‐vitro transcription translation of FSP27 and VEGF‐A followed by immunoblot of FSP27 depicting an interaction between FSP27 and VEGF‐A. C, Western blot showing that siRNA (small interfering RNA)‐mediated inhibition of FSP27 nearly fully abolished VEGF‐A in human aortic endothelial cells. Experiments were repeated 3 times. FSP27 indicates fat‐specific protein 27; IP, immunoprecipitation; IB, immunoblot; VEFG‐A, vascular endothelial growth factor‐A.