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. 2019 May 25;8(11):e011260. doi: 10.1161/JAHA.118.011260

Figure 8.

Figure 8

Protein kinase A catalytic subunit (PKAc) phosphorylation (pPKAc) and O‐GlcNAcylation in early hypertrophy. Immunoblots for phosphorylated PKAc at Thr197 normalized to total PKAc in the indicated early hypertrophy groups (A through C). Immunoprecipitation for PKAc with subsequent immunoblotting for O‐linked β‐N‐acetylglucosamine (O‐GlcNAc) and PKAc (for normalization) in early hypertrophy (D). In (D) input lane 1 is the whole cell lysate from control transverse aortic constriction (TAC) and input lane 2 is the whole cell lysate from O‐GlcNAc transferase knockout (OGTKO) TAC. n=7 per group for A–C. n=3 per group in (D). Values are arbitrary units reported as mean±standard error of the mean. *P<0.05 between groups indicated with the bar. @ P value from t test=0.058 and P value from Tukey procedure=0.57 between the groups indicated with the bar. For (D) control sham vs OGTKO sham, P value from t test=0.028 and P value from Tukey procedure=0.20. WB indicates Western blot.