Skip to main content
. Author manuscript; available in PMC: 2020 Jul 1.
Published in final edited form as: Wiley Interdiscip Rev RNA. 2019 Apr 15;10(4):e1534. doi: 10.1002/wrna.1534

Figure 4. Model for enhancement of OAS1 activation by RNA sequence and structural motifs.

Figure 4.

OAS1 requires at least ~17 bp dsRNA for binding, shown here on the left for the model 18 bp dsRNA duplex with OAS1 consensus activation sequence (purple) and right the nc886 central stem sequence. Once positioned by the OAS1 binding site, RNA signatures such as the 3’-ssPy motif (orange) or the tertiary structure of nc886 (red) can dramatically increase OAS1 activation. The mechanism of activation by these RNA features is currently unknown but modeling and mutagenesis analyses suggest the OAS1 loop containing G157 may be important (see main text for details).