KEY RESOURCES TABLE

REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
GFP	Life Technologies	Cat # A11122; RRID: AB_221569
nc82	DSHB	AB_2314866
TH	Chemicon	Cat # MAB318; RRID: AB_2201528
Goat anti rabbit – Alexa 488	Life Technologies	Cat # A11008; RRID: AB_143165
Goat anti mouse – Alexa 633	Life Technologies	Cat # A21052; RRID: AB_2535719
Deposited Data
Raw original data	This paper	https://doi.org/10.17632/nssxb2fmmt.1
Experimental Models: Organisms/Strains
DTH-C’-Gal4,F-Gal80	Mark Wu Lab	Liu et al., 2012b
DTH-D’-Gal4	Mark Wu Lab	Liu et al., 2012b
TH-Gal4	Ron Davis Lab	Friggi-Grelin et al., 2003
Oc2-Gal4	Jorge Blanco	Blanco et al., 2011
NP3024-Gal4	Kyoto Stock Center DGRC	#113066
NP5945-Gal4	Kyoto Stock Center DGRC	#105062
murashka-1-Gal4	Josh Dubnau Lab	Dubnau et al., 2003
UAS-TrpA1	Paul Garrity Lab	Hamada et al., 2008
MB-GCaMP3	Seth Tomchik Lab	Boto et al., 2014
UAS-mCD8::GFP	Bloomington Drosophila Stock Center	#32195
Software and Algorithms
ImageJ		https://imagej.nih.gov/ij/
MATLAB 2015b	MathWorks	https://www.mathworks.com/products/matlab.html
Prism 6.07	GraphPad	https://www.graphpad.com/
Leica Application Suit X (LASX)	Leica Microsystems	https://www.leica-microsystems.com