Table 5:

Complex III assay flow-chart

REAGENTS	BLANK	SAMPLE (−) AntimycinA	SAMPLE (+) AntimycinA
(1) Reaction Buffer for Complex-III	225 μL	227.5 – x μL	225 – x μL
(2) 50 mM KCN	10 μL	10 μL	10 μL
(3) 1.25 mM Cyt c (oxd.)	10 μL	10 μL	10 μL
(4) 1 mM AntimycinA	2.5 μL	-	2.5 μL
(5) Protein solution	-	X μL	X μL
(6) Incubate the plate with a gentle mixing for 10 at 30°C
(7) Start the reaction by adding 5 mM DBH2	2.5 μL	2.5 μL	2.5 μL
(8) Measure the absorbance at A550nm for 2–3 minutes