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Complex IV assay flow-chart
| REAGENTS | BLANK | SAMPLE (−) KCN |
SAMPLE (+) KCN |
|---|---|---|---|
| (1)O.1 M KP Buffer (pH 7.4) | 230 μL | 240 μL– | 230 μL –xμL |
| (2)Protein solution | - | X μL | X μL |
| (3)50 mM KCN | 10 μL | - | 10 μL |
| (3) Start the reaction by adding 0.5 mM Cyt-c (reduced) | 10 μL | 10 μL | 10 μL |
| (4) Measure the absorbance at A550nmfor 10 min | |||
