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Citrate synthase assay flow-chart
| REAGENTS | BLANK | SAMPLE |
|---|---|---|
| (1) Water | 197.5 μL | 197.5 μL – x μL |
| (2) Reaction mixture for citrate synthase reaction | 40 μL | 40 μL |
| (3) Protein solution Incubate the plate for 5 min at 30°C | - | X μL |
| (4) Start the reaction by adding 10 mM oxaloacetic acid | 12.5 μL | 12.5 μL |
| (5) Measure the absorbance at A412nm for 3–4 min. | ||
