Skip to main content
. Author manuscript; available in PMC: 2020 Feb 1.
Published in final edited form as: J Immunol. 2018 Dec 28;202(3):920–930. doi: 10.4049/jimmunol.1800226

Figure 1. IRF5 is required for optimal intracellular bacterial clearance.

Figure 1.

(A) Timeline schematic for M1 polarization, IRF5 knockdown and LPS treatment. (B) MDMs (n=6 donors, similar results in n=12–16) were left untreated (M0) or polarized into M1 macrophages and then transfected with scrambled or IRF5 siRNA. Cells were then left untreated or treated with 0.1μg/ml LPS for 48h and co-cultured with E. faecalis, AIEC, S. aureus or S. Typhimurium. Colony forming units (CFU)+SEM. Significance is compared to scrambled siRNA-transfected cells for each respective condition (i.e. M0, M1 and M1+LPS) or as indicated. Scr, scrambled; **, p<0.01; ***, p<0.001; †, p<1×10−4; ††, p<1×10−5.