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. Author manuscript; available in PMC: 2020 Feb 1.
Published in final edited form as: J Immunol. 2018 Dec 28;202(3):920–930. doi: 10.4049/jimmunol.1800226

Figure 7. Rs2004640/rs2280714 genotype-dependent regulation of bacterial clearance, ROS, RNS and autophagy proteins is due to IRF5 expression modulation.

Figure 7.

MDMs from rs2004640/rs2280714 TT/TT or GG/CC carriers (n=15/genotype) were transfected with 25 nM scrambled or IRF5 siRNA (A-E), or empty vector or IRF5 vector (F-J), and then polarized to M1 macrophages and treated with 0.1μg/ml LPS for 48h. (A,F) Summarized MFI of IRF5 as assessed by flow cytometry+SEM. (B,G) Cells were cultured with E. faecalis or S. Typhimurium. CFU+SEM. (C-E, H-J) Summarized MFI of ROS or the indicated proteins as assessed by flow cytometry+SEM. NS, not significant; scr, scrambled. ††, p<1×10−5.